ELECTROPHORETIC STUDIES OF CONVERSION PRODUCTS OF SERUM BETA1C-GLOBULIN

Abstract

By the method of antigen-antibody crossed electrophoresis where the antigens after a first separation in agarose gel are driven into a new antibody containing gel, [human] serum [beta]1c-globulin and its conversion products were analysed in native serum as well as after addition of EDTA, incubation with immunoprecipitate and hydrazine, and after inactivation at 56[degree]. It is shown that [beta]1c-globulin can be converted not only to [beta]1A-globulin or components with the same mobility as [beta]1A-globulin but also to additional com-ponents, one in the [alpha]2-region, one in-the inter-b-region and one in the [gamma]1-region. [alpha]2- and inter-[beta]-components are seen especially in serum to which EDTA has been added. EDTA did not inhibit the spontaneous conversion of [beta]1c-globulin.