This study was designed to examine the relationship between attitudes toward homosexuality and attitudes toward certain aspects of heterosexual sexuality and to personal sex guilt and sex stereotyping. One hundred twenty-six Canadian male students completed a series of specially devised attitude scales pertaining to these areas. On the basis of their responses to the anti-homosexual scale, subjects were divided into a prohomosexual (Pro-H) and antihomosexual (Anti-H) group. Results showed that Anti-H respondents were more intolerant of a variety of heterosexual behaviors. Anti-H Ss reported more personal sex-guilt and higher level of repression of their own sexual impulses than did Pro-H subjects. The Anti-H group also demonstrated a greater stereotyping of the sexes by their sex-typing of a variety of personality characteristics, hobbies, and professions. These Ss were also more willing to label a man as homosexual when he exhibited what they thought of as a single feminine characteristic than were Pro-H subjects. These findings provide supportive evidence for the theoretical formulations of Churchill (4) regarding antihomosexualism in a sex-negative environment. As the intercorrelations between the three principal attitude scales were only moderate, it appears that attitudes toward homosexuality are at least somewhat specific, not just part of a more general sexual attitude. Similarly, anti-homosexual attitudes correlate positively but moderately with general sexual conservatism and with personal sex guilt. Discussion of these moderate correlations suggested the relevance of various sources of prejudice. The uncritical acceptance of culturally pervasive stereotypes could account for much measured antihomosexual feelings. It was also suggested that development of the culturally acceptable masculine identity is facilitated by the individual's desire to avoid the stigmatization of being “feminine;; which, in cultural terms, means also being “queer.”