Reaction Mechanism of the ATPase Activity of Mitochondrial Fi Studied by Using a Fluorescent ATP Analog, 2'-(5-Dimethyl-aminonaphthalene-1-Sulfonyl) Anuno-2'-DeoxyATP: Its Striking Resemblance to That of Myosin ATPase1

Abstract
The reaction mechanism of beef heart mitochondrial F1-ATPase was studied by using a fluorescent ATP analog, 2'-(5-dimethylaminonaphthalene-1-sulfonyl) amino-2'-deoxyATP (DNS-ATP), as a substrate. The following results were obtained.