Production and specificity of monoclonal antibodies to retinal S antigen

Abstract

Hybridomas producing monoclonal antibodies to the retinal S antigen were obtained by fusion of spleen cells from a BALB/c mouse immunized with purified bovine S antigen and NS-1 myeloma cells. Six cloned hybridomas were selected and expanded as large scale culture and as ascites in mice. The specificity of the antibodies produced by these hybridomas was assessed by ELISA [enzyme linked immunosorbent assay] and immunofluorescence. All were specific for S antigen, except one which showed slight reactivity with other proteins. One antibody was specific for bovine S antigen, whereas the others showed cross reactivity with purified S antigens from various mammals. Immunofluorescence allowed demonstration of the presence of common epitopes of S antigen in the retinal photoreceptor cells of species representative of every class of Vertebrates: mammals (man, cat, rabbit, guinea pig, rat and mouse); birds (Gallus); reptiles (lizard, Vipera and Pseudemys); amphibians (Pleurodeles, Xenopus and Rana); teleost (Salmo and Carassius); and selachians (Scylliorhinus and Torpedo).