D-Ribulose-1,5-bisphosphate carboxylase and polyhedral inclusion bodies in Thiobacillus intermedius
- 1 July 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 127 (1), 516-522
- https://doi.org/10.1128/jb.127.1.516-522.1976
Abstract
The growth-related parameters of T. intermedius, cultured in glutamate-CO2-S2O32- medium, were determined. After centrifugation at 48,000 .times. g for 1 h, 24% of the D-ribulose-1,5-bisphosphate carboxylase (RuBPCase) activity of the disrupted-cell suspensions obtained from CO2-S2O32-- and glutamate-CO2-S2O32--grown cells could be sedimented and the specific activities of this enzyme in the supernatant fractions were almost equivalent. The enzyme was stable in T. intermedius starved of thiosulfate in the presence and absence of glutamate, but a progressive decrease was evident in several growth cycles, each cycle supported by resupplementation of cells with thiosulfate. Polyhedral inclusion bodies were present in CO2-S2O32-- and glutamate-CO2-S2O32--grown cells. The number of polyhedral bodies per cell increased during mixotrophic growth approximately in proportion to the observed increase in the specific activity of RuBPCase. RuBPCase could not be detected in T. intermedius grown heterotrophically on yeast extract, nor could polyhedral bodies be found.This publication has 25 references indexed in Scilit:
- [69] Ribulose-diphosphate carboxylase from the hydrogen bacteria and Rhodospirillum rubrumMethods in Enzymology, 1975
- Ultrastructure of a Marine ThiobacillusJournal of General Microbiology, 1974
- D-ribulose-1,5-diphosphate carboxylase and the evolution of autotrophyBiosystems, 1974
- Mechanisms of Inorganic Oxidation and Energy CouplingAnnual Review of Microbiology, 1974
- Fine structure of selected species of the genus Thiobacillus as revealed by chemical fixation and freeze-etchingCanadian Journal of Microbiology, 1974
- On the sulfur-source requirement for growth of Thiobacillus intermediusArchiv für Mikrobiologie, 1974
- Functional Organelles in Prokaryotes: Polyhedral Inclusions (Carboxysomes) of Thiobacillus neapolitanusScience, 1973
- The Roles of Exogenous Organic Matter in the Physiology of Chemolithotrophic BacteriaPublished by Elsevier ,1969
- Thiobacillus intermedius nov.sp.Archiv für Mikrobiologie, 1963
- Eine vollsynthetische N hrl sung zur selektiven Anreicherung einiger SchwefelpurpurbakterienThe Science of Nature, 1961