Quantitation of mRNAs during mouse spermatogenesis: protamine-like histone and phosphoglycerate kinase-2 mRNAs increase after meiosis.
- 1 October 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (10), 6086-6090
- https://doi.org/10.1073/pnas.77.10.6086
Abstract
Total RNA, prepared from immature or mature mouse testes or from spermatogenic cells separated on the basis of sedimentation velocity, was translated in vitro. Mouse protamine-like histone could be identified as an in vitro translational product when [3H]arginine was used as the label. The mRNA for protamine-like histone was detected only after meiosis; the appearance of a peak of radioactivity comigrating with protamine-like histone occurred only when RNA from mature testes or late spermatid cell fractions was translated. Phosphoglycerate kinase-2 (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) was identified as an in vitro translational product by affinity chromatography, followed by 2-dimensional gel electrophoresis or by specific immunoprecipitation when [35S]methionine was the label. The mRNA for phosphoglycerate kinase-2 was detected only in mature testes or late spermatid cell fractions. These translational assays for protamine-like histone and phosphoglycerate kinase-2 mRNA suggest that these messages are transcribed after meiosis.This publication has 33 references indexed in Scilit:
- Variation among inbred strains of mice in adenosine 3′:5′ cyclic monophosphate levels of spermatozoaGenetics Research, 1979
- Protamine messenger RNA: Evidence for early synthesis and accumulation during spermatogenesis in rainbow troutDevelopmental Biology, 1978
- Identification and isolation of protamine messenger ribonucleoprotein particles from rainbow trout testisBiochemistry, 1977
- Testis specific phosphoglycerate kinase B in mouseJournal of Experimental Zoology, 1976
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- The expression of β-glucuronidase during preimplantation development of mouse embryosDevelopmental Biology, 1976
- Synthesis and amino acid composition of basic proteins in mammalian sperm nucleiDevelopmental Biology, 1975
- Centrifugal elutriation: Separation of spermatogenic cells on the basis of sedimentation velocityJournal of Cellular Physiology, 1975
- The biosynthesis of protamine during spermatogenesis of the mouse: Extraction, partial characterization, and site of synthesisJournal of Cellular Physiology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970