A Quantitative Study of The Gibberellin Content of Seeds of Phaseolus Vulgaris at Different Stages in Their Development

Abstract

Extracts were made, using ethyl acetate, from bean seeds at different stages of development. Chromatograms of the extracts were developed using n-butanol-l' 5N ammonium hydroxide (3 : 1 vjv) and the chromatograms surveyed by two methods of bioassay for gibberellin activity. Activity was found in two zones, zone 1 at RF 0'3-0'4, zone 2 at RF 0,7-0,9. The amount of activity in each zone (compared with that of known amounts of gibberellic acid) changes in step with changes in the growth rate of the seeds. The bean seed has a diauxic pattern of growth.