Determination of Thiopental by High Pressure Liquid Chromatography
- 1 April 1979
- journal article
- research article
- Published by Wiley in Acta Pharmacologica et Toxicologica
- Vol. 44 (4), 260-263
- https://doi.org/10.1111/j.1600-0773.1979.tb02327.x
Abstract
A specific and rapid reverse phase HPLC[high pressure liquid chromatography]-method for the determination of thiopental in serum and in protein free solutions is described. No extraction is used. The detection limit is about 0.090 .mu.g/ml for serum and about 0.030 .mu.g/ml for buffer solutions. The retention time is 3.80 min. No interference is found with drugs commonly used in patients with heart diseases. The method was used to follow the serum concentrations in 7 patients who were given thiopental as an introduction to general anesthesia.Keywords
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