Calcium Channel, Ca++ mobilization, and Mechanical Reactivity of Estrogen- and Progesterone-Treated Rat Uterus
Open Access
- 1 January 1986
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 41 (1), 47-54
- https://doi.org/10.1254/jjp.41.47
Abstract
Properties of [3H]nitrendipine binding, high K+- and Ca2+-induced contractions and the inhibition of high K+-induced contractions by verapamil were investigated in the uterine preparations isolated from rats treated with estrogen or progesterone or both. In [3H]nitrendipine binding experiments using crude membrane fractions, treatment with estrogen alone or estrogen + progesterone significantly lowered the KD; There was very little change in the Bmax. In the Ca2+-depleted, high K+-containing medium, only the progesterone-, and estrogen .fwdarw. progesterone-treated uteri produced contractions. The estrogen-, estrogen .fwdarw. progesterone-, and estrogen + progesterone-treated uteri showed decreases in concentrations of Ca2+ required for the maximal contractions. In the estrogen- and estrogen + progesterone-treated uteri, the dose-response curves by verapamil were shifted to the left in a parallel manner. These findings suggest that estrogen appeared to increase the affinity of calcium channels and increse transmembrane influx of Ca2+, leading to enhancement of contractions, whereas progesterone might increase the Ca2+ storage in the intracellular sites.Keywords
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