Bacterial diversity of subgingival plaque in 6 healthy Chinese individuals
Open Access
- 30 June 2011
- journal article
- Published by Spandidos Publications in Experimental and Therapeutic Medicine
- Vol. 2 (5), 1023-1029
- https://doi.org/10.3892/etm.2011.311
Abstract
The subgingival microbial ecology is complex, and little is known regarding its bacteria species composition in healthy Chinese individuals. This study aimed to identify the subgingival microbiota from 6 healthy Chinese subjects. Subgingival samples from 6 volunteers were collected, the 16S rRNA gene was amplified using broad-range bacterial primers, and clone libraries were constructed. For the initial 2,439 sequences analyzed, 383 species-level operational taxonomic units (SLOTUs) belonging to seven phyla were identified, estimated as 51% [95% confidence interval (CI) 44–55] of the SLOTUs in this ecosystem. Most (85%) of the bacterial sequences, falling into 228 types of species, corresponded to known and cultivated species. However, 146 (6%) sequences, comprising 104 phylotypes, had <97% similarity to prior database sequences. Ten bacterial genera were conserved among all 6 individuals, comprising 2,000 (82%) of the 2,439 clones analyzed. Ten species were noted in all of the 6 subjects, comprising 1,435 (58.8%) of the 2,439 clones. Streptococcus infantis was the species most frequently cloned. Furthermore, certain species which may participate in the pathogenesis of periodontal disease were present in the 6 subjects. Although the initial subgingival plaque community of each subject was unique in terms of diversity and composition, 10 common key species were found in the 6 Chinese individuals. These ten species of bacteria in the human subgingival plaque in the 6 healthy individuals may be key species which, to some extent, affect periodontal health. Destruction of these key species in subgingival bacteria may break the microbiota balance and may easily lead to over-breeding conditions resulting in pathogenic oral disease.Keywords
This publication has 24 references indexed in Scilit:
- Bacterial diversity in the oral cavity of 10 healthy individualsThe ISME Journal, 2010
- Comparisons of Subgingival Microbial Profiles of Refractory Periodontitis, Severe Periodontitis, and Periodontal Health Using the Human Oral Microbe Identification MicroarrayThe Journal of Periodontology, 2009
- Diversity and site-specificity of the oral microflora in the elderlyEuropean Journal of Clinical Microbiology & Infectious Diseases, 2009
- Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young AdultsJournal of Clinical Microbiology, 2008
- Molecular analysis of human forearm superficial skin bacterial biotaProceedings of the National Academy of Sciences, 2007
- Oral Probiotics Prevent Necrotizing Enterocolitis in Very Low Birth Weight NeonatesThe Journal of Pediatrics, 2005
- Introducing DOTUR, a Computer Program for Defining Operational Taxonomic Units and Estimating Species RichnessApplied and Environmental Microbiology, 2005
- Bellerophon: a program to detect chimeric sequences in multiple sequence alignmentsBioinformatics, 2004
- From dissimilarities among species to dissimilarities among communities: a double principal coordinate analysisJournal of Theoretical Biology, 2004
- Detection of novel oral phylotypes associated with periodontitisFEMS Microbiology Letters, 2002