Myrosinase inSinapis albaL.

Abstract

Extracts from developing seeds, seedings, and different organs of mature plants of Sinapis alba L. were tested for the presence of myrosinase. Three different patterns of isoenzymes were distinguished by isoelectric focusing. One was found in developing seeds and seedlings, a second in mature leaves, stems, flowers, and pods and a third in roots. Sections of all the parts tested were examined for the presence of myrosin cells by light microscopy. Developing seeds and seedlings showed a good correlation between the appearance of myrosinase and the presence of myrosin cells. No myrosin cells were found in root or flower parts although they showed myrosinase activity.