Sedimentation of Spermatozoa and Settling of Diluter Solids and their Effects upon Survival of Spermatozoa during Storage

Abstract

The visible settling of egg yolk and semen plasma solids during storage or shipping of diluted bovine semen has little or no effect upon duration of sperm motility or survival. Maintaining spermatozoa in suspension by the use of gelatin or other inert viscous material did not prolong viability. The sedimentation rate for live and dead cells was almost equal at the usual storage temp. The sample should be thoroughly mixed prior to utilization or evaluation.