Microcapsule Modification with Peroxidase-Catalyzed Phenol Polymerization
- 2 March 2004
- journal article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 5 (3), 914-921
- https://doi.org/10.1021/bm034476x
Abstract
A biocatalytic polymer synthesis on a surface of polyelectrolyte microcapsules was studied. Horseradish peroxidase assembled in nanoorganized capsule walls by alternate adsorption with linear polyions retains its activity in reactions of enzyme-catalyzed polymerization of 4-oxyphenols. It allowed controllable synthesis of a phenolic polymer layer on microcapsule walls using an outermost surface peroxidase layer as a template. By varying the phenol type, buffer pH, and reaction component concentrations, the phenolic polymer coating of the capsules with a thickness in the range 20−50 nm was formed. The polymeric products are fluorescent, which provided a good opportunity for confocal image analysis of the capsule wall structure and the attached layer. The influence of a phenolic polymer layer on the permeability of the capsule walls was investigated.Keywords
This publication has 14 references indexed in Scilit:
- Urease‐Catalyzed Carbonate Precipitation inside the Restricted Volume of Polyelectrolyte CapsulesMacromolecular Rapid Communications, 2003
- Urease Encapsulation in Nanoorganized MicroshellsNano Letters, 2001
- Enzyme Encapsulation in Layer-by-Layer Engineered Polymer Multilayer CapsulesLangmuir, 2000
- Biologically Derived Conducting and Water Soluble PolyanilineMacromolecules, 1998
- Direct Electrochemistry of Myoglobin and Cytochrome P450cam in Alternate Layer-by-Layer Films with DNA and Other PolyionsJournal of the American Chemical Society, 1998
- Molecularly Flat Films of Linear Polyions and Proteins Obtained by the Alternate Adsorption MethodJapanese Journal of Applied Physics, 1997
- Enzymatic Synthesis and Characterization of a Novel Water-Soluble Polyaniline: Poly(2,5-diaminobenzenesulfonate)Macromolecules, 1997
- Polymer Precipitation Using a Micellar Nonsolvent: The Role of Surfactant−Polymer Interactions and the Development of a Microencapsulation TechniqueIndustrial & Engineering Chemistry Research, 1996
- Assembly of Multicomponent Protein Films by Means of Electrostatic Layer-by-Layer AdsorptionJournal of the American Chemical Society, 1995
- Homovanillic acid as a fluorometric substrate for oxidative enzymes. Analytical applications of the peroxidase, glucose oxidase, and xanthine oxidase systemsAnalytical Chemistry, 1968