Opsonic activity in human serum chelated with ethylene glycoltetra-acetic acid.
- 1 June 1974
- journal article
- Vol. 26 (6), 1251-6
Abstract
The influence of the alternative pathway of complement activation on opsonization of S. aureus, E. coli and Candida albicans for phagocytosis was selectively studied by chelating serum with 10 mM EGTA and 10 mM MgC12. Opsonic activity of the chelated serum depended on the alternative pathway of complement activation. The classical pathway was inhibited. There was no phagocytosis of S. aureus in chelated normal serum, suggesting that activation of complement activity by the classical pathway is necessary for opsonization of this micro-organism. In control experiments when opsonization of S. aureus was accomplished by IgG separated from hyperimmune serum, without the requirement of complement activity, there was no inhibition of phagocytosis by EGTA and magnesium chloride. E. coli and C. albicans were opsonized in chelated normal serum, indicating that the alternative pathway of complement activation is involved in opsonization of these micro-organisms.This publication has 18 references indexed in Scilit:
- Bactericidal and Opsonic Properties of C4-Deficient Guinea Pig SerumThe Journal of Immunology, 1972
- Opsonic Activity in Human Serum Deficient in C2The Journal of Immunology, 1972
- Quantitation of Staphylococcal Protein A: Determination of Equilibrium Constant and Number of Protein a Residues on BacteriaThe Journal of Immunology, 1970
- HEAT LABILE OPSONINS TO PNEUMOCOCCUSThe Journal of Experimental Medicine, 1969
- Defective candidacidal capacity of polymorphonuclear leukocytes in chronic granulomatous disease of childhoodThe Journal of Pediatrics, 1969
- Studies of an Additional Kindred with Hereditary Deficiency of the Second Component of Human Complement (C2) and Description of a New Method for the Quantitation of C2The Journal of Immunology, 1968
- Hereditary Deficiency of the Second Component of Complement (C′2) in Man: Further Observations on a Second KindredThe Journal of Immunology, 1967
- COMPLEMENT-DEPENDENT BIOLOGICAL FUNCTIONS IN COMPLEMENT DEFICIENCY IN MANThe Lancet, 1966
- Hereditary deficiency of the second component of complement (C'2) in man.JCI Insight, 1966