Characteristics of PR5, a lipid-containing plasmid-dependent phage
- 1 July 1978
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 24 (7), 875-882
- https://doi.org/10.1139/m78-145
Abstract
An extensive characterization of plasmid-dependent phage PR5 isolated from sewage has been carried out. The phage has a head diameter of 65–68 nm, is isometric with a double-layered capsid, and a minority possess tails. It adsorbs to many but not all types of bacteria possessing P, N, or W plasmids. The phage contains 20% lipid, 15.1% DNA, and 64.9% protein by weight and has a buoyant density of 1.265 g/ml in CsCl. The DNA is double-stranded with a G + C content of 49% and a molecular weight of 7.4 ± 0.6 × 106 as shown by electron microscopy. Phospholipid content is 66% of the lipid and consists of cardiolipin (13%), phosphatidylethanolamine (43%), and phosphatidylglycerol (44%) and differs quantitatively from that of host bacteria. Anti-PR5 serum inactivates other similar phages, PR3 and PR4. Phage adsorption is impaired in deep rough mutants of Salmonella minnesota.This publication has 5 references indexed in Scilit:
- Adsorption of the R-specific Bacteriophage PR4 to Pili Determined by a Drug Resistance Plasmid of the W Compatibility GroupJournal of General Microbiology, 1976
- Lipid and Protein Arrangement in Bacteriophage PM2Nature New Biology, 1971
- Properties of bacteriophage PM2: A lipid-containing bacterial virusVirology, 1968
- ANTIBIOTIC SUSCEPTIBILITY TESTING BY A STANDARDIZED SINGLE DISK METHOD1966
- Sedimentation studies of the size and shape of DNAJournal of Molecular Biology, 1965