Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12
- 1 September 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 187 (1), 101-106
- https://doi.org/10.1007/bf00384390
Abstract
In vitro recombination techniques were used to clone the E. coli metJBLF gene cluster in a plasmid vector. Several chimeric plasmids were obtained, analyzed by restriction mapping and characterized genetically. The combined results establish that the met gene cluster is contained on an approximately 5.6 kilobase segment of bacterial DNA with metL between metB and metF. The origin of metL was localized precisely by its DNA sequence and its transcription direction was established.This publication has 43 references indexed in Scilit:
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