Nucleotide sequence ofIS110, an insertion sequence ofStreptomyces coelicolorA3(2)
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (17), 7053-7085
- https://doi.org/10.1093/nar/15.17.7053
Abstract
The nucleotide sequences of the Streptonyces transposable element IS110 and its insertion site in the DNA of a derivative of the temperate phage πC31 were determined. The element is inserted about 460 bp from the right-hand end of φC31 DNA, in a region of apparently non-coding DNA. The target site (in a run of seven C residues) is within an 11 bp sequence homologous with one end of IS110. The inserted element is flanked by runs of 11 and 15 C residues which form part of more extensive regions of homology between the left and right junction regions. Imperfect inverted repeats (10 matches out of 15 bp) are present near (but not at) the ends of IS110. The whole IS110 element contains about 1550 bp of which 71% are G-C bp. One major potentially protein-coding region (ORF 1215) was detected, of 1215 bp, the product of which, a presumptively soluble protein of MR, 43, 563, was not overtly related to any entry in a protein sequence database. A smaller open reading frame (ORF 330) was tentatively identified in the opposite strand of the ORF 1215 region.Keywords
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