Distribution of pyrimidine bases in herring-roe deoxyribonucleic acid
- 1 December 1955
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 61 (4), 529-534
- https://doi.org/10.1042/bj0610529a
Abstract
Herring-roe DNA was partly degraded to pyrimidine polynucleotides by non-enzymic methods. The purines were selectively removed and the liberated reducing sugars were blocked in the acylic form as ethylmercaptals. The thymine/ cytosine ratio of the product differed little from that of the original DNA. The mercaptalated product was hydrolyzed by dilute alkali to yield diffusible organo-phosphorus compounds, and a non-diffusible purine-free substance containing thymine and cytosine. The degradation is consistent with the "elimination" mechanism of nucleotide breakdown. The results suggest that there are regions in DNA where at least three pyrimidine nucleotides are linked together.Keywords
This publication has 13 references indexed in Scilit:
- DISTRIBUTION DENSITY OF NUCLEOTIDES WITHIN A DESOXYRIBONUCLEIC ACID CHAINJournal of Biological Chemistry, 1953
- THE IDENTITY OF THE PURINE-BOUND PENTOSE OF SOME STRAINS OF TOBACCO MOSAIC VIRUSJournal of Biological Chemistry, 1953
- The enzymic breakdown of deoxyribosenucleic acidsBiochimica et Biophysica Acta, 1952
- Polynucleotides from Deoxyribonucleic AcidsNature, 1952
- THE CHROMATOGRAPHIC IDENTIFICATION OF SOME BIOLOGICALLY IMPORTANT PHOSPHATE ESTERSJournal of Biological Chemistry, 1951
- The purine and pyrimidine composition of deoxypentose nucleic acidsBiochemical Journal, 1951
- PURINE AND PYRIMIDINE CONTENTS OF SOME DESOXYPENTOSE NUCLEIC ACIDSThe Journal of general physiology, 1950
- Separation of the Phosphoric Esters on the Filter Paper ChromatogramNature, 1949
- Chromatographic studies of nucleic acids. 1. A technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substancesBiochemical Journal, 1949
- The estimation of phosphorusBiochemical Journal, 1940