COMPARATIVE SELECTIVITY OF ADJUVANTS FOR HUMORAL AND CELL-MEDIATED-IMMUNITY .2. EFFECT ON DELAYED-TYPE HYPERSENSITIVITY IN THE MOUSE AND GUINEA-PIG, AND CELL-MEDIATED-IMMUNITY TO TUMOR-ANTIGENS IN THE MOUSE OF FREUNDS INCOMPLETE AND COMPLETE ADJUVANTS, ALHYDROGEL, CORYNEBACTERIUM-PARVUM, BORDETELLA-PERTUSSIS, MURAMYL DIPEPTIDE AND SAPONIN

Abstract

C. parvum [Propionibacterium acnes] was the only adjuvant of those tested which consistently potentiated delayed-type hypersensitivity (DTH) to sheep red blood cells (SRBC) in the mouse, although this required the antigen-adjuvant mixture to be injected s.c. in the footpad rather than the flank. For induction of DTH to ovalbumin (OVA) in the guinea pig, C. parvum and MDP [muramyl dipeptide] could be substituted for the mycobacteria in FCA. C. parvum was effective in aqueous solution provided that the OVA was adsorbed onto alhydrogel. Saponin also potentiated DTH when injected with OVA in aqueous solution. C. parvum was the sole adjuvant of those tested to promote protective cell-mediated immunity (CMI) to M4 fibrosarcoma cells. C. parvum is a promising candidate adjuvant for promoting CMI induction without recourse to a water-in-oil emulsion.