Oxidative Stress and Tumour Cell Proliferation
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in Free Radical Research Communications
- Vol. 11 (1-3), 65-76
- https://doi.org/10.3109/10715769009109669
Abstract
The effects of oxidant stress were studied in immortalised hamster (BHK-21) and rat (208F) cell lines before and after transformation to the malignant state with polyoma virus, or activated H-ras, respectively. Whilst intracellular superoxide production was detectable in both transformed and immortalised cells the rate was somewhat higher in the transformed cells which have lower levels of superoxide dismutase. Because growth of transformed cells was particularly depressed in the presence of MTT, a tetrazolium compound reduced by superoxide, the possible role of active oxygen species in the promotion of cell growth was examined. Low levels of hydrogen peroxide were stimulatory towards both immortalised and transformed cells. In the case of H-ras transformed rat cells, paraquat was also stimulatory provided serum was present in the growth medium. In the absence of serum, paraquat was notably inhibitory but inhibition could be alleviated by addition of low concentrations of α-tocopherol (10−8 M) to the serum-depleted medium. Although depletion of serum from the growth medium also leads to lower cell proliferation, subsequent experiments showed that a-tocopherol addition to serum-free medium was sufficient to restimulate growth. In the case of transformed cells, yields of cells were even greater than that encountered in the presence of 10% serum. Thus whilst certain active oxygen species (e. g. hydrogen peroxide) may have a role in promoting the growth of transformed and immortalised cells the necessity for antioxidant protection is important.Keywords
This publication has 24 references indexed in Scilit:
- Cell Proliferation and Oxidative StressFree Radical Research Communications, 1989
- The Molecular Genetics of CancerScience, 1987
- Growth stimulation of rat primary embryo fibroblasts by the human myc geneExperimental Cell Research, 1986
- Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenesNature, 1984
- Transformation of NIH 3T3 cells by microinjection of Ha-ras p21 proteinNature, 1984
- Amplification of specific DNA sequences correlates with multi-drug resistance in Chinese hamster cellsNature, 1984
- Viral and cellular fos proteins: A comparative analysisCell, 1984
- Serum stimulates na entry and the Na‐K pump in quiescent cultures of epithelial cells (MDCK)Journal of Cellular Physiology, 1983
- The roles of individual polyoma virus early proteins in oncogenic transformationNature, 1982
- Human heat shock gene expression and the modulation of plasma membrane Na+,K+‐ATPase activityFEBS Letters, 1982