Biochemical analysis of meiosis in the male mouse
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 62 (3), 243-253
- https://doi.org/10.1007/bf00286046
Abstract
Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of semi-conservative meiotic DNA synthesis, essential to the biochemical analysis of pachytene DNA metabolism, has been achieved by in vivo administration of 1 M hydroxyurea.This publication has 7 references indexed in Scilit:
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