Shape and Functional Properties of Human Platelets Washed with Acid Citrate
- 1 January 1981
- journal article
- research article
- Published by S. Karger AG in Pathophysiology of Haemostasis and Thrombosis
- Vol. 10 (1), 14-27
- https://doi.org/10.1159/000214383
Abstract
Acid citrate pH 6.5 protects human platelets from activation during washing at room temperature. Platelets suspended in media containing citrate pH 6.5 have an extra-flat disc shape. Citrate at pH 6.5 also inhibits the ADP-induced shape change. When warmed to 37.degree. C and suspended into the test medium of pH 7.4, the washed platelets aggregate with low concentrations of ADP or epinephrine in the presence of fibrinogen and Ca2+. The morphological and functional quality of platelet concentrates (6 .times. 106 .mu.l-1) stored in acid citrate pH 6.5 is maintained for at least 6 h at room temperature.This publication has 12 references indexed in Scilit:
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