Formation of Adenosine 5′-Phosphorofluoridate and the Assay of Adenyl Cyclase in Barley Seeds
- 1 February 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 53 (2), 144-148
- https://doi.org/10.1104/pp.53.2.144
Abstract
Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5′-phosphorofluoridate from magnesium-adenosine 5′-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3′: 5′-monophosphate was detected in barley seed homogenates or extracts.Keywords
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