Production and Purification of the Extracellular Ribonuclease of Bacillus Amyloliquefaciens (Barnase) and its Intracellular Inhibitor (Barstar) II. Barstar

Abstract

Procedures are reported for the extraction and purification of barstar, the intracellular inhibitor of barnase, the extracellular ribonuclease of Bacillus amyloliquefaciens, (a strain formerly erroneously classified as B. subtilis, strain H), from frozen acidified cells obtained during the isolation of barnase¹. Methods include affinity chromatography on agarose to which barnase is covalently bound. Experiments relating to the purity, molecular weight, and amino acid composition of the product are reported.