Amino acids and peptides XXXI. Phosphorus in organic synthesis XVIII. Synthesis of porcine motilin by the solid-phase method using diphenyl phosphorazidate(DPPA) and diethyl phosphorocyanidate(DEPC).

Abstract

Synthesis of porcine motilin, which exhibits a gastric motor stimulating activity, was accomplished by the solid-phase method with diphenyl phosphorazidate (DPPA) and diethyl phosphorocyanidate (DEPC) as coupling reagents. The purified synthetic peptide showed characteristic contractile activity towards the duodenum, colon and jejunum in the rabbit. Its potency was almost the same as that of the natural material.