Evidence against the occurrence of adenosine-3?:5?-cyclic monophosphate in higher plants
- 1 January 1974
- journal article
- Published by Springer Nature in Planta
- Vol. 118 (3), 241-258
- https://doi.org/10.1007/bf00384780
Abstract
Previous reports on the incorporation of [14C]adenine into adenosine-3′:5′-cyclic monophosphate (cyclic AMP) in oat (Avena sativa L.) and maize (Zea mays L.) coleoptile sections, chick-pea (Cicer arietinum L.) embryos and barley (Hordeum vulgare L.) aleurone layers were reexamined. Separation of labelled nucleotides on DEAE-Sephadex A 25 showed that a peak of 14C activity, previously considered to be cyclic AMP, is not identical with this compound. Attempts to detect the cyclic nucleotide by means of a highly specific protein-kinase assay in various plant tissues (Nicotiana tabacum L., tissue culture; Catharanthus roseus Don., tissue culture; Lycopersicon esculentum Mill, seedlings; Nicotiana tabacum, pith parenchyma; Avena sativa, coleoptiles) failed even though up to 100 g of plant material was extracted and a number of control experiments were carried out to insure that cyclic AMP, if present in the extracts, could be measured.Keywords
This publication has 37 references indexed in Scilit:
- Cyclic nucleotide phosphodiesterases in plantsZeitschrift für Pflanzenphysiologie, 1974
- Cyclic Nucleotide Phosphodiesterase Activity in Barley SeedsPlant Physiology, 1973
- Evidence that Cyclic AMP Does Not Mediate the Action of Gibberellic AcidNature, 1973
- Inability to detect cyclic AMP in vegetative or sporulating cells or dormant spores of BacillusmegateriumBiochemical and Biophysical Research Communications, 1973
- Cyclic Purine Mononucleotides: Induction of Gibberellin Biosynthesis in Barley EndospermPhysiologia Plantarum, 1972
- Studies on the Mechanism of Hormone ActionScience, 1972
- Die wirkung von cyclischem adenosin-3′,5′-monophosphat auf das streckungswachstum von Avena-ColeoptilzylindernZeitschrift für Pflanzenphysiologie, 1972
- Rapid gibberellin responses and the action of adenosine 3′,5′-monophosphate in aleurone layersBiochimica et Biophysica Acta (BBA) - General Subjects, 1971
- Auxin-induced synthesis of cyclic 3′, 5′-adenosine monophosphate in coleoptilesLife Sciences, 1971
- Effect of indole-3-acetic acid on the synthesis of cyclic 3′–5′ adenosine phosphate by bengal gram seedsBiochemical and Biophysical Research Communications, 1971