Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA
- 1 January 1985
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 92 (3), 225-233
- https://doi.org/10.1007/bf00348698
Abstract
The human Y chromosome contains a group of repeated DNA elements, identified as 3.4-kilobase pair (kb) fragments in Hae III digests of male genomic DNA, which contain both Y-specific and non-Y-specific sequences. We have used these 3.4-kb Hae III Y fragments to explore the organizational properties and chromosomal distribution of the autosomal homologs of the non-Y-specific (NYS) 3.4-kb Hae III Y elements. Three distinct organizations, termed domains, have been identified and shown to have major concentrations on separate chromosomes. We have established that domain K is located on chromosome 15 and domain D on chromosome 16 and suggested that domain R is on chromosome 1. Our findings suggest that each domain is composed of a tandemly arrayed cluster of a regularly repeating unit containing two sets of repeated sequences: one that is homologous to the NYS 3.4-kb Hae III Y sequences and one that does not cross-react with the 3.4-kb Hae III Y repeats. Thus, these autosomal repeated DNA domains, like their Y chromosome counterparts, consist of a complex mixture of repeated DNA elements interspersed among each other in ways that lead to defined periodicities. Although each of the three identified autosomal domains cross-reacts with 3.4-kb Hae III Y fragments purified from genomic DNA, the length periodicities and sequence content of the autosomal domains are chromosome specific. The organizational properties and chromosomal distribution of these NYS 3.4-kb Hae III homologs seem inconsistent with stochastic mechanisms of sequence diffusion between chromosomes.Keywords
This publication has 46 references indexed in Scilit:
- Distribution of mitomycin C induced breaks on human chromosomesHereditas, 2009
- Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosomeJournal of Molecular Biology, 1985
- Dispersion of the ras family of transforming genes to four different chromosomes in manNature, 1983
- Structural organization of α-satellite DNA in a single monkey chromosomeJournal of Molecular Biology, 1982
- Molecular drive: a cohesive mode of species evolutionNature, 1982
- Sequence definition and organization of a human repeated DNAJournal of Molecular Biology, 1980
- A study of sequence homologies in four satellite DNAs of manJournal of Molecular Biology, 1979
- Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acidBiochemistry, 1979
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Isolation of the complementary strands of a human satellite DNAJournal of Molecular Biology, 1968