RELIABILITY OF A DISK DIFFUSION METHOD USING SEMICONFLUENT GROWTH IN THE DETERMINATION OF AMINOGLYCOSIDE RESISTANCE
- 1 September 1986
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Series B: Microbiology
- Vol. 94B (1-6), 153-157
- https://doi.org/10.1111/j.1699-0463.1986.tb03035.x
Abstract
Susceptibility of 553 blood culture isolates to gentamicin, tobramycin, amikacin, and netilmicin was determined by a routine disk diffusion method with semiconfluent growth (AB Biodisk, Solna, Sweden) and a microtiter-method (Sensititre). The disk diffusion test gave false sensitive results in 9.4-71% of the 9-39 aminoglycoside resistant strains studied. False resistant results occurred in 1.0-7.6% of measurements among the over 500 aminoglycoside sensitive strains. Because of the frequent error rate, the disk method with semiconfluent growth should be replaced with other methods in the determination of aminoglycoside resistance in bacteriological laboratories.Keywords
This publication has 14 references indexed in Scilit:
- Comparison of antibiotic susceptibility results obtained with Adatab and disc methods.Journal of Clinical Pathology, 1984
- Error rates associated with the use of recently proposed breakpoints for testing Pseudomonas aeruginosa versus gentamicin, tobramycin, and amikacin by the standardized disk agar diffusion testAntimicrobial Agents and Chemotherapy, 1983
- Antimicrobial susceptibility tests: for better or worsePathology, 1983
- Clinical Impact of Rapid Antimicrobial Susceptibility Testing of Blood Culture IsolatesAntimicrobial Agents and Chemotherapy, 1982
- A Revised System for Antibiotic Sensitivity TestingScandinavian Journal of Infectious Diseases, 1981
- An Analysis of Error Rates for Disc Agar-diffusion Testing of Pseudomonas aeruginosa Versus AminoglycosidesAmerican Journal of Clinical Pathology, 1981
- Comparison of sensititre dried microtitration trays with a standard agar method for determination of minimum inhibitory concentrations of antimicrobial agentsAntimicrobial Agents and Chemotherapy, 1980
- Antibiotic Susceptibility Testing of Pseudomonas aeruginosa: Selection of a Control Strain and Criteria for Magnesium and Calcium Content in MediaThe Journal of Infectious Diseases, 1974