Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells

Abstract

Identification of biomaterials that support appropriate cellular attachment, proliferation and gene expression patterns is critical for tissue engineering and cell therapy. Here we describe an approach for rapid, nanoliter-scale synthesis of biomaterials and characterization of their interactions with cells. We simultaneously characterize over 1,700 human embryonic stem cell–material interactions and identify a host of unexpected materials effects that offer new levels of control over human embryonic stem cell behavior.