Incorporation of Glycine Into Protein Fractions of Nuclei of Liver and Hepatoma.

Abstract
A procedure is described for the separation of the proteins of cell nuclei into several fractions which differ in solubilities and in glycine content. Twenty hours after intraperitoneal injection of glycine-1-C14 into adult rats bearing subcutaneously transplanted hepatomas, the specific activities of the protein fractions of the nuclei of liver and tumor were in the descending order: residual lipoprotein globulins > histone fraction III > histone fraction II. Each of the protein fractions of the nuclei of liver cells had higher specific activities than corresponding protein fractions of the hepatoma with the notable exception of the histones which were in the reverse relationship.