The complex pattern of cytokines in serum from patients with meningococcal septic shock. Association between interleukin 6, interleukin 1, and fatal outcome.

Abstract

Serum samples from patients with meningococcal disease were examined for the presence of IL-6, TNF-.alpha., and LPS. Median serum concentration of IL-6 was 1,000 times higher in patients with septic shock (189 ng/ml) than in patients with bacteriaemia, meningitis, or combined septic shock and meningitis. 11 of 21 patients with serum levels > 3.0 ng/ml died, whereas all 58 patients with serum levels at .ltoreq. 3.0 ng/ml, survived. All four patients with serum IL-6 levels > 750 ng/ml, died. IL-1 was detected in serum from three patients who also had high serum levels of IL-6, TNF-.alpha., and LPS, and rapidly fatal courses. IL-6 appeared to be released into serum later than TNF-.alpha., and was detected in serum for up to 36 h. The half-life of IL-6 and TNF-.alpha. was calculated to be 103 .+-. 27 min and 70 .+-. 11 min, respectively. These data indicate that a complex pattern of cytokines exists in serum from patients with meningococcal septic shock, and that the release of IL-6 and IL-1, in addition to TNF-.alpha., is associated with fatal outcome.