Immunophoretic Evaluation of Blood Serum Proteins in Chickens. I. Changing Protein Patterns in Chickens According to Age.

Abstract

Summary and Conclusions 1. Immuno-phoresis was performed in a series of 42 normal chickens from ages 1 to 210 days. Rabbit serum immunized against normal mature chicken serum was used for antibodies. Bar-bital buffer at pH 8.6, ionic strength 0.1 was used. 2. Identification of fractions was attempted by designating precipitation curves in the chick serum preparation with the names used for curves of known fractions in identical position and of identical shape in human serum preparations made under identical conditions. 3. Significant findings in chicken serum indicate that all fractions are more abundant in neonatal life than at later periods. 4. Prealbumin becomes less abundant after the 12th day of life, and is not detected in the preparations after the 20th day. 5. Precipitation curve in the position of the beta lipoprotein fraction is not observed in preparations after the 8th day of life. 6. Additional fractions of gamma globulin appear in some preparations after the 12th day of life. 7. The findings are significant for comparison with serological protein profiles of chickens on vit E-deficient diets.