Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

Abstract
The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli , 17 of 43 (39.5%) Klebsiella pneumoniae , 3 of 50 (6.0%) Enterobacter cloacae , and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.