OBSERVATIONS ON THE USE OF TETRAZOLIUM SALTS IN THE VITAL STAINING OF BACTERIA
- 1 June 1959
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 5 (3), 245-250
- https://doi.org/10.1139/m59-028
Abstract
Studies on the vital staining of bacteria were carried out to determine the concentrations of tetrazolium salts required for optimal staining, and the amounts which could cause complete inhibition of bacterial growth. Four salts were studied: neotetrazolium chloride (NTC), triphenyltetrazolium chloride (TTC), blue tetrazolium (BT), and nitro-blue tetrazolium (NBT). Evidence has been presented to show that only living, actively metabolizing cells can reduce neotetrazolium to formazan.Keywords
This publication has 1 reference indexed in Scilit:
- TETRAZOLIUM REDUCTION TEST AS A MEASURE TO EXAMINE THE TOTAL VIABILITY OF SUSPENSIONS OF TUBERCLE BACILLI I. STUDIES ON THE TECHNIOUES OF THE TESTJapanese Journal of Medical Science and Biology, 1954