Differentiation of Arthrobacter soil isolates and named strains from other bacteria by reactions on dye-containing media

Abstract

Twenty-five gram-negative and 62 gram-positive bacterial cultures, of which 37 werearthrobacters, were tested, using a multipoint inoculation device, for responses on varying concentrations of 35 dyes. Both selective (growth vs. no growth) and differential (dye absorption) responses were obtained. It was possible to differentiate the arthrobacters from the other gram-positive bacteria tested as well as to distinguish separately each Arthrobacter-named strain. The results indicated that dyes have a greater potential for use in selective and differential media than has been presently realized; and the possibility of using dye-reactions as features in taxonomic schemes to differentiate rapidly many of the common genera of soil bacteria is discussed.