Amino acid sequence of a variant pro-form of insulin-like growth factor II.
- 1 May 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (10), 3169-3172
- https://doi.org/10.1073/pnas.82.10.3169
Abstract
Human serum contains, in addition to the classical 7.5-kDa [kilodalton] insulin-like growth factors (IGF) I and II, small amounts of larger IGF-II. A 10-kDa IGF-II was isolated by gel filtration, immunoaffinity chromatography, and reversed-phase HPLC [high performance liquid chromatography]. Upon amino acid sequence determination, a substitution of Cys-Gly-Asp for Ser-33 was found as well as a COOH-terminal extension of 21 residues (E peptide). These sequence differences suggest that 10-kDa IGF-II is a precursor of a variant IGF-II. Since the substitution is not located at a known intron/exon hinge region, the finding of this variant IGF-II is evidence for the presence of more than 1 gene for IGF-II.This publication has 20 references indexed in Scilit:
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