Effect of Photoperiod on the Metabolism of Deuterium-Labeled Gibberellin A53 in Spinach

Abstract
Application of GA53 to short day(SD)-grown spinach (S. oleracea L.) plants caused an increase in petiole length and leaf angle similar to that found in plants transferred to long days (LD). [2H]GA53 was fed to plants in SD, LD and in a SD to LD transition experiment, and the metabolites were identified by gas chromatography with selected ion monitoring. After 2, 4 or 6 SD, [2H]GA53 was converted to [2H]GA19 and [2H]GA44. No other metabolites were detected. After 2 LD, only [2H]GA20 was identified. In the transition experiment in which plants were given 4 SD followed by 2 LD, all 3 metabolites were found. GA19, GA20 and GA44 are metabolic products of GA53, photoperiod apparently regulates GA metabolism in part by controlling the conversion of GA19 to GA20.