Glucosyltransferase activity in a water extract of maize pollen
- 1 January 1972
- journal article
- Published by Springer Nature in Planta
- Vol. 103 (4), 361-364
- https://doi.org/10.1007/bf00386709
Abstract
Glucosyltransferase activity was extracted from maize pollen in distilled water. The enzymatic reaction required UDP-glucose, mercaptoethanol and Ca++, and had a pH optimum at 8.2. Either kampferol or quercetin served as a substrate. Michaelis-Menten constants obtained were 0.6×10-4 M for quercetin and 0.74×10-3M for UDP-glucose. Ammonium-sulfate precipitation of the enzyme gave a 4fold purification.Keywords
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