Activation of Membrane-Bound Adenylate Cyclase by Glucagon in Neurospora crassa

Abstract
Membrane-bound adenylate cyclase in Neurospora crassa is activated by glucagon. Half-maximal effect is observed at hormone concentrations of about 10 nM. After solubilization of the enzyme with Lubrol-PX, the glucagon effect is lost. Incubation of neurospora cells with glucagon leads to a decrease in the activity of glycogen synthetase (EC 2.4.1.11) and to an increase in the activity of glycogen phosphorylase (EC 2.4.1.1) and in the rate of glycogenolysis.