A Method for the Superfusion of Rat Hypothalami: Secretion of Luteinizing Hormone-Releasing Hormone (LH-RH)

Abstract

Summary A superfusion system for hy-pothalamic tissue is reported and the secretion rate of LH-RH is analyzed under different experimental conditions. The responsiveness of the preparation to stimuli like high K⁺, low Ca²⁺, and prostaglandin E₂ was assessed. The usefulness of this preparation as an in vitro system to study the secretion rate of hypothalamic hormones is discussed. The authors wish to thank Mrs. Robyn Luke for her skillful secretarial assistance and Dr. J. Pike from Upjohn Company for providing us generously with prostaglandins.