Intermediates in the folding of the membrane protein bacteriorhodopsin
- 1 February 1995
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 2 (2), 139-143
- https://doi.org/10.1038/nsb0295-139
Abstract
Assembly of proteins within lipid bilayers is essential for the biogenesis and function of biological membranes. Little is known, however, about the underlying mechanism of assembly, and it is not clear whether it is possible to observe individual folding steps for integral membrane proteins either in vivo or in vitro. Fluorescence spectroscopy is used here to follow the time course of folding events for bacteriorhodopsin in mixed detergent/lipid micelles. Transient folding-intermediates are detected and binding of the retinal chromophore occurs at a late stage, when it binds to an apoprotein intermediate.Keywords
This publication has 31 references indexed in Scilit:
- Seven-helix receptorsCurrent Opinion in Structural Biology, 1991
- Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopyJournal of Molecular Biology, 1990
- Reconstitution of pigment-containing complexes from light-harvesting chlorophyll a/b-binding protein overexpressed inEscherichia coliPlanta, 1990
- Membrane protein folding and oligomerization: the two-stage modelBiochemistry, 1990
- Structure and thermal stability of monomeric bacteriorhodopsin in mixed pospholipid/detergent micellesProteins-Structure Function and Bioinformatics, 1989
- Refolding of bacteriorhodopsin in lipid bilayersJournal of Molecular Biology, 1987
- The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesisCell, 1981
- Binding of all-trans-retinal to the purple membrane. Evidence for cooperativity and determination of the extinction coefficientBiochemistry, 1979
- Studies on the Retinal-Protein Interaction in BacteriorhodopsinEuropean Journal of Biochemistry, 1977
- Reversible Dissociation of the Purple Complex in Bacteriorhodopsin and Identification of 13‐cis and all‐trans‐Retinal as its ChromophoresEuropean Journal of Biochemistry, 1973