Rapid and specific immunoassay for cardiac troponin I in the diagnosis of myocardial damage
- 1 March 1997
- journal article
- research article
- Published by Springer Nature in International Journal of Clinical and Laboratory Research
- Vol. 27 (1), 60-64
- https://doi.org/10.1007/bf02827244
Abstract
We evaluated a new immunometric assay for the quantitation of cardiac troponin I with the Behring Opus analyzer. All assay steps are performed automatically by the analyzer, the complete procedure requiring 20 min for the first test result. Total precision (coefficient of variation) varied between 5.6% and 13.0%. Comparison with the Pasteur immunoenzymometric assay showed good correlation (r=0.959), but the Opus assay gave approximately tenfold higher values. Cardiac troponin I was undetectable (<0.5 Μg/1) in sera from healthy subjects (n=46), patients with severe skeletal muscle damage (n=8), and all but 1 patient with chronic renal failure (n=39). In patients with myocardial infarction (n=21), investigated for 4 days after onset by frequent sampling, cardiac troponin I peaked at 20.8±8.1 h after onset with a mean concentration of 164.8±361.3 Μg/l, remaining elevated in 89% of patients on the 4th day. In patients with unstable angina (n=15), cardiac troponin I measurement was valuable in predicting the type of lesion morphology on coronary angiography and the short-term outcome.Keywords
This publication has 8 references indexed in Scilit:
- Nonspecific elevation of troponin T levels in patients with chronic renal failureClinical Biochemistry, 1995
- Cardiac troponin I. A marker with high specificity for cardiac injury.Circulation, 1993
- Cardiac troponin I to diagnose myocardial injuryThe Lancet, 1993
- Lesion morphology and coronary angioplasty: Current experience and analysisJournal of the American College of Cardiology, 1992
- Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles.Molecular and Cellular Biology, 1988
- Cardiac-specific troponin-l radioimmunoassay in the diagnosis of acute myocardial infarctionAmerican Heart Journal, 1987
- Nomenclature and criteria for diagnosis of ischemic heart disease. Report of the Joint International Society and Federation of Cardiology/World Health Organization task force on standardization of clinical nomenclature.Circulation, 1979
- Comparison of amino acid sequence of troponin I from different striated musclesNature, 1978