Hepatic regeneration and metabolism after partial hepatectomy in normal rats: effects of insulin therapy
- 1 October 1986
- journal article
- research article
- Published by Wiley in European Journal of Clinical Investigation
- Vol. 16 (5), 376-383
- https://doi.org/10.1111/j.1365-2362.1986.tb01012.x
Abstract
The effect of insulin therapy on liver regeneration has been studied in normal fed rats 12, 24 and 48 h after partial hepatectomy. Dry weight of regenerating liver increased between 12 and 48 h after partial hepatectomy and was unaffected by insulin therapy. [6‐3H] Thymidine uptake peaked at 24‐h (24·7 ± 2·4% of total liver cells) and insulin treatment had no additional effect. At 12‐h after partial hepatectomy, hepatic [ATP] was decreased 15%, while [ADP] and [AMP] were increased 47% and 83% respectively compared with sham‐operated animals. Partial hepatectomy also caused an increase in hepatic [triglyceride], a decrease in hepatic [glycogen] and an increase in the levels of glucose and several glycolytic intermediates. The hepatic redox ratios, [lactate]:[pyruvate] and [3‐hydroxybutryate]:[acetoacetate], were elevated. Insulin therapy had only minor effects on hepatic adenine nucleotide levels, intermediary metabolite concentrations or intrahepatic redox ratios after partial hepatectomy. These findings suggest a decreased hepatic intracellular energy state in regenerating liver; insulin therapy in normal rats does not influence this metabolic change nor the regenerative response.Keywords
This publication has 26 references indexed in Scilit:
- Protein synthesis in regenerating liverInternational Journal of Biochemistry, 1980
- Metabolic Changes in the Serum of Partially Hepatectomized RatsHormone and Metabolic Research, 1980
- Increased effect of adrenaline on cyclic AMP formation and positive β‐adrenergic modulation of DNA‐synthesis in regenerating hepatocytesFEBS Letters, 1980
- Correlation between Rates and Enzyme Levels of Increased Gluconeogenesis in Rat Liver and Kidney after Partial HepatectomyEuropean Journal of Biochemistry, 1979
- The biochemistry of the regenerating liverInternational Journal of Biochemistry, 1977
- Insulin Requirements for Hepatic Regeneration Following HepatectomyAnnals of Surgery, 1977
- Glucose intolerance after massive liver resection in man and other mammalsThe American Journal of Surgery, 1975
- GROWTH CONTROL OF DIFFERENTIATED FETAL RAT HEPATOCYTES IN PRIMARY MONOLAYER CULTUREThe Journal of cell biology, 1974
- Influence of Protracted Infusion of Glucose and Insulin on the Composition and Regeneration Activity of Liver after Partial Hepatectomy in RatsNature, 1967
- Eine neue Bestimmung der Neutralfette im Blutserum und GewebeKlinische Wochenschrift, 1966