Subunit association and heterogeneity of Limulus polyphemus hemocyanin
- 18 April 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (8), 1448-1455
- https://doi.org/10.1021/bi00601a014
Abstract
The MW of the 6S, 24S, 36S and 60S components of L. polyphemus hemocyanin were determined by high speed sedimentation equilibrium to be 69,400, 856,000, 1,690,000 and 3,160,000. The behavior of this hemocyanin appears similar to that of other arthropod hemocyanins where the 1st aggregation step is the formation of a hexamer of the 6S monomer. Here the larger aggregated states (24S, 36S and 60S) are successive dimers of an unobserved hexamer (16S). The 24S-36S-60S association was heterogeneous, suggesting that 24S components of different composition may be present.This publication has 8 references indexed in Scilit:
- Equilibrium centrifugation of proteins in acid solutionsArchives of Biochemistry and Biophysics, 1976
- Hemocyanin of the horseshoe crab, Limulus polyphemus. Structural differentiation of the isolated components.Journal of Biological Chemistry, 1976
- Use of gel chromatography for the determination of the Stokes radii of proteins in the presence and absence of detergents. A reexaminationBiochemistry, 1976
- Isolation and characterization of a hemagglutinin from Limulus polyphemusJournal of Molecular Biology, 1968
- Macromolecular organization of hemocyanins and apohemocyanins as revealed by electron microscopyJournal of Molecular Biology, 1966
- Equilibrium Ultracentrifugation of Dilute Solutions*Biochemistry, 1964
- THE SEDIMENTATION CONSTANTS OF THE RESPIRATORY PROTEINSThe Biological Bulletin, 1934
- Die elementare Zusammensetzung verschiedener Hämocyanine.Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1933