Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40‐transformed B cells
- 9 March 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 213 (1), 149-154
- https://doi.org/10.1016/0014-5793(87)81481-3
Abstract
In HIT-T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co-migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2–20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose-dependent manner. Glucose-stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.Keywords
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