Partial purification of rat α-lactalbumin mRNA
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (6), 2065-2074
- https://doi.org/10.1093/nar/4.6.2065
Abstract
α-Lactalbumin messenger RNA was partially purified from RNA extracted from 3–5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. α-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat α-lactalbumin. In the purified mRNA preparation a-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.Keywords
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