Adenosine Triphosphatase Activity Associated with Purified Cholinergic Synaptic Vesicles of Torpedo marmorata
- 1 October 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 80 (1), 313-318
- https://doi.org/10.1111/j.1432-1033.1977.tb11884.x
Abstract
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromatography on CPG-10-3000 porous glass beads. Synaptic vesicles have a buoyant density of 1.057 g/ml. Purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+,K+-stimulated ATPase activity. They contain a ouabain-insensitive Na+,K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline.This publication has 34 references indexed in Scilit:
- Some physical properties of adrenal medulla chromaffin granules isolated by a new continuous iso-osmotic density gradient methodBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- THE BINDING OF ACETYLCHOLINE TO SYNAPTIC VESICLES FROM TORPEDO CALIFORNICA ELECTROPLAXJournal of Neurochemistry, 1976
- ACETYLCHOLINE TRANSLOCATION IN SYNAPTIC VESICLE GHOSTS IN VITROJournal of Neurochemistry, 1976
- The preparation and characterization of synaptic vesicles of high purityBrain Research, 1976
- Nerve terminal ATPase as possible trigger for neurotransmitter releaseNature, 1975
- Removal of residual amounts of acetylcholinesterase and membrane contamination from synaptic vesicles isolated from the electric organ of TorpedoJournal of Neurochemistry, 1973
- Magnesium-dependent ATP-ase in membranes of 5-hydroxytryptamine storage organellesBiochemical and Biophysical Research Communications, 1972
- ATP Hydrolysis and Noradrenaline Transport in Purified Vesicles from Splenic Nerve TrunkActa Physiologica Scandinavica, 1971
- COMPOSITION OF CELLULAR MEMBRANES IN THE PANCREAS OF THE GUINEA PIGThe Journal of cell biology, 1971
- The influence of some cations on an adenosine triphosphatase from peripheral nervesBiochimica et Biophysica Acta, 1957