Increase of glucose and lactate output and decrease of flow by human anaphylatoxin C3a but not C5a in perfused rat liver
- 16 January 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 243 (1), 83-87
- https://doi.org/10.1016/0014-5793(89)81222-0
Abstract
The complement fragments C3a and C5a were purified from zymosan-activated human serum by column chromatographic procedures after the bulk of the proteins had been removed by acidic polyethylene glycol precipitation. In the isolated in situ perfused rat liver C3a increased glucose and lactate output and reduced flow. Its effects were enhanced in the presence of the carboxypeptidase inhibitor DL-mercaptomethyl-3-guanidinoethylthio-propanoic acid (MERGETPA) and abolished by preincubation of the anaphylatoxin with carboxypeptidase B or with Fab fragments of an anti-C3a monoclonal antibody. The C3a effects were partially inhibited by the thromboxane antagonist BM13505. C5a had no effect. It is concluded that locally but not systemically produced C3a may play an important role in the regulation of local metabolism and hemodynamics during inflammatory processes in the liver.Keywords
This publication has 18 references indexed in Scilit:
- Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and factor BKlinische Wochenschrift, 1988
- Leukotrienes increase glucose and lactate output and decrease flow in perfused rat liverBiochemical and Biophysical Research Communications, 1988
- Effects of Leukotrienes and the Thromboxane A2Analogue U-46619 in Isolated Perfused Rat Liver. Metabolic, Hemodynamic and Ion-Flux ResponsesBiological Chemistry Hoppe-Seyler, 1988
- Hepatocyte heterogeneity in response to extracellular ATPEuropean Journal of Biochemistry, 1987
- Possible involvement of eicosanoids in the zymosan and arachidonic‐acid‐induced oxygen uptake, glycogenolysis and Ca2+ mobilization in the perfused rat liverEuropean Journal of Biochemistry, 1987
- Possible involvement of prostaglandins in vasoconstriction induced by zymosan and arachidonic acid in the perfused rat liverFEBS Letters, 1987
- Effects of the anaphylatoxin, C5a, on renal and glomerular hemodynamics in the ratKidney International, 1986
- Direct Control of Glycogen Metabolism in the Perfused Rat Liver by the Sympathetic InnervationEuropean Journal of Biochemistry, 1982
- A potent mercapto bi-product analogue inhibitor for human carboxypeptidase NBiochemical and Biophysical Research Communications, 1981
- Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidaseJCI Insight, 1970