Studies on the Inorganic Requirements of Tetrahymena

Abstract

T. geleii W in pure-culture (bacteria-free) were grown on a chemically defined medium. By omission expts., requirements for K, Mg, and phosphate were shown. No requirement for Ca could be demonstrated either by omission expts. or by the use of citrate or oxalate. No requirement for trace elements could be demonstrated in the medium used, which probably means that some or all of the crystalline amino acids used in the medium were contaminated.