Double-Stranded Ribonuclease Coinduced with Interferon
- 2 June 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 244 (4908), 1089-1091
- https://doi.org/10.1126/science.2471268
Abstract
Double-stranded (ds) RNA and many viruses are inducers of interferon (IFN), the latter presumably because they contain, or can form, dsRNA. Concomitant with the induction of IFN in chicken embryo cells was the induction of a novel double-stranded ribonuclease (dsRNase), which was released into the medium and continued to accumulate long after IFN production ceased. Only avian cells (chicken, quail, turkey, or duck) expressed high levels of this dsRNase; mammalian, turtle, or fish cells did not. Production of the nuclease was inducer dose-dependent. Optimum pH and cation requirements distinguished it from other dsRNase activities. Degradation of dsRNA was endonucleolytic. Activity resided in a molecule of an Mr of approximately 34,500. Low levels of a single-stranded (ss) RNase activity were inseparable from the dsRNase. The role for a dsRNA-inducible dsRNase released from cells is unknown.This publication has 32 references indexed in Scilit:
- Interferon Induction by Viruses. XVII. Non-temperature-sensitive Mutations Regulate Interferon Induction by Vesicular Stomatitis VirusJournal of General Virology, 1989
- INTERFERONS AND THEIR ACTIONSAnnual Review of Biochemistry, 1987
- Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activityCell, 1987
- Interferon Induction by Viruses. X. A Model for Interferon Induction by Newcastle Disease VirusJournal of General Virology, 1983
- Interferon Induction by Viruses. VI. Reovirus: Virion Genome dsRNA as the Interferon Inducer in Aged Chick Embryo CellsJournal of Interferon Research, 1980
- Cell surface‐associated ribonuclease activitiesJournal of Cellular Physiology, 1978
- Open states in native polynucleotides: II. Hydrogen-exchange study of cytosine-containing double helicesJournal of Molecular Biology, 1975
- Dimeric structure of seminal ribonucleaseFEBS Letters, 1972
- The geometry of nucleic acidsProgress in Biophysics and Molecular Biology, 1970
- Polynucleotides. VI. Molecular properties and conformation of polyribouridylic acidBiopolymers, 1963